Pyruvate kinase blood test
Pyruvate Kinase Blood Test
A Pyruvate Kinase (PK) blood test is used to assess a person’s enzyme activity levels in their red blood cells. This enzyme helps to convert glycolytic intermediate products such as phosphoenolpyruvate into other metabolic intermediates. It is known to be involved in several different metabolic pathways such as the glycolytic pathway, Krebs cycle, and pentose phosphate pathway.
Preparation
In order to have a Pyruvate Kinase blood test, the patient needs to fast for at least 12 hours before the test is conducted. This will ensure that the results are accurate.
Procedure
A small sample of blood is collected from the patient, usually from the arm. The blood is then analyzed in a laboratory to determine the activity levels of Pyruvate Kinase in the red blood cells.
Types of Pyruvate Kinase Test
Pyruvate Kinase tests are typically done as part of a metabolic assessment panel to assess enzyme activity levels in the red blood cells.
- Pyruvate Kinase 1 (PK1)
- Pyruvate Kinase 2 (PK2)
- Pyruvate Kinase 3 (PK3)
Risks Involved
There are no risks associated with a Pyruvate Kinase blood test as the blood is taken from a small vein in the arm. The patient might experience slight pain and bruising due to the needle prick.
When to Get Tested
A Pyruvate Kinase blood test is typically ordered when an individual has symptoms of anemia, such as fatigue or pale skin. It can also be ordered when someone has a family history of hereditary hemolytic anemia or if they have been diagnosed with a blood disorder.
Why Test is Performed
Pyruvate Kinase helps to convert glycolytic intermediate products into other metabolic intermediates. As such, a Pyruvate Kinase blood test is used to assess for deficient activity of this enzyme, which can lead to a wide range of metabolic disorders. A decrease in enzyme activity can cause anemic conditions, including hereditary hemolytic anemia.