Immunofixation (IFE) Blood Test

What is Immunofixation (IFE) Blood Test?

Immunofixation (IFE) Blood Test is a blood test used to identify monoclonal protein present in serum or urine. It is also referred to as Immunoelectrophoresis, and is used to diagnose monoclonal gammopathy and multiple myeloma.

Why Immunofixation (IFE) Blood Test is done?

An Immunofixation (IFE) test can help diagnose monoclonal gammopathy (MG) and multiple myeloma (MM). MG is an abnormal protein detected in the blood or urine, which is usually associated with a disease in the bone marrow. MM is a cancer of the plasma cells. It is important to diagnose and treat early to prevent further complications.

Preparation for the Test

No special preparation is needed for an Immunofixation (IFE) test. You simply need to fast for a period of time before the test, and to not take any medications until after the test is done.

Procedure for the Test

The Immunofixation (IFE) test involves collecting and analyzing a blood or urine sample. The sample is analyzed using specialized equipment and reagents to determine the presence or absence of monoclonal antibody.

Types of Test

There are two types of Immunofixation (IFE) tests. The first is the gel electrophoresis test. This test involves running a gel containing the sample through an electric field. Monoclonal IgG, IgA, and IgM antibodies will be visible on the gel as they pass through the electric field. The second is the immunofixation antibody test. This test involves incubating the sample with labeled antibodies, which will then bind to the monoclonal antibody if present in the sample.


There are no risks associated with the Immunofixation (IFE) test. The only risk would be if the sample was contaminated in some way.

When and Why

The Immunofixation (IFE) test is generally used as part of a diagnostic workup to identify monoclonal proteins in serum or urine. It is usually ordered when a person has symptoms of monoclonal gammopathy or multiple myeloma. It can also be used to monitor treatment for patients with these conditions.